Abstract
OBJECTIVE To develop a reversed phase high performance liquid chromatographic method for simultaneous determination of four tanshinones in rat plasma in the pharmacokinetic study.METHODS The assay was conducted on a VP-ODS C18 column(4.6 mm×250 mm,5 μm),and the mobile phase consisted of methanol and 0.05 mol·L-1 ammonium acetate buffers volume ratio of 70∶30(pH 2.5).The flow rate was 1.0 mL·min-1.The detection wavelength was set at 263 nm.Testosterone propionate was used as internal standard.RESULTS The response was linear in the concentration range of 0.01～12.75 mg·L-1 for cryptotanshinone,0.01～16.25 mg·L-1 for tanshinoneⅠ 0.02～13.75 mg·L-1 for methltanshinone and 0.02～13.25 mg·L-1 for tanshinone ⅡA,respectively.The recovery of assay was above 88.7%.The pharmacokinetic paramenters showed that cryptotanshinone and tanshinoneⅡafter oral administration were fitted with a two-compartment open model while the methltanshinone and tanshinone Ⅰ were fitted with one-compartment open model.CONCLUSION The method was simple and reliable and could be used to simultaneously determine the four kinds of tanshinones in rat plasma.
Key words
RP-HPLC /
cryptotanshinone /
tanshinoneⅠ /
methltanshinone /
tanshinoneⅡA /
pharmacokinetic
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DU Wei;WEI Yu-hui;ZHO Gng;QIN Hong-yn;WU Xin-n.
Simultaneous Determination of Four Tanshinones in Rat Plasma by High Performance Liquid Chromatography and Its Application to Pharmacokinetic Studies [J]. Chinese Pharmaceutical Journal, 2008, 43(10): 786-789
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