Effects of Shaoqiduogan on Rats with Collagen-Induced Arthritis
WEI Fang，WEI Wei
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Institute of Clinical Pharmacology，Anhui Medical University，Anhui Key Lab of Antiinflammatory and Immunopharmacology，Anhui Center of Engineering for Antiinflammatory and Immune Drugs，Anhui Key Lab of Research and Development for Chinese Medicine，Hefei 230032，China
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History+
Received
Published
2007-07-05
2008-05-10
Issue Date
2008-05-10
Abstract
OBJECTIVE To study the treatment effects of Shaoqiduogan(SQDG) on collagen-induced arthritis(CIA) in rats.METHODS Chicken type Ⅱ collagen was used to induce CIA in rats.The volume of uninduced-inflammation paws of rats,arthritic scores and tenderness of uninduced-inflammation paws were determined for evaluating occurrence status of arthritis.The diversity of thymus index and spleens index were observed.T and B cells proliferation was determined by 3-(4,5-dimethylthiazal-2yl) 2,5-diphenyltetrazoli-umbromide(MTT) assay,and interleukin-1(IL-1) activity was measured by thymocyte proliferation assay.Anti-Ⅱ collagen antibody in blood was detected by ELISA assay.RESULTS Rats occurrenced red swelling of the paws on d 14 after immunity,crest-time of secondary inflammation was on d 22,and the inflammation began abatement on d 26.SQDG(21.25,42.5,85 mg·kg-1),TGP(50 mg·kg-1),ASTs(7 mg·kg-1)ig alleviated the inflammation of joint at different degree and decreased thymus index,spleens index of CIA rats.The further study demonstrated that SQDG,TGP and ASTs suppressed the excessive T and B cell proliferation and over-production of IL-1 and interleukin-2(IL-2),and decreased the elevated anti-Ⅱ collagen antibody in blood.CONCLUSION SQDG has a treating effect on CIA in rats,and the mechanism of treatment maybe concern with its regulation on the abnormal immune responses in CIA rats.
WEI Fng;WEI Wei.
Effects of Shaoqiduogan on Rats with Collagen-Induced Arthritis [J]. Chinese Pharmaceutical Journal, 2008, 43(10): 746-750
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