Comparison of Syringin Content Between Cell Cultures and Botanical Drug of Saussurea involucrata
LI Yan，GUO Shun-xing，WANG Chun-lan*，WU Li-qin，XIAO Pei-gen
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Institute of Medical Plant Development，Chinese Academy of Medicinal Sciences and Peking Union Medical College，Beijing 100094，China
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Published
2008-05-10
Issue Date
2008-05-10
Abstract
OBJECTIVE To compare the syringin content in cell cultures with that in botanical drug of Saussurea involucrata.METHODS A HPLC-DAD method was developed as follows:chromatographic separation was achieved on an Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm) at 25 ℃ and the mobile phase was a mixture of acetonitrile-water(10∶90) detected at 220 nm at a flow rate of 1.0 mL·min-1.RESULTS Compared with the botanical drug,the content of syringin in cells was improved 20.96 times by liquid culture and 55.50 times by solid culture.CONCLUSION It has a good prospect to acquire syringin by plant cell culture.
LI Yn;GUO Shun-xing;WNG Chun-ln;WU Li-qin;XIO Pei-gen.
Comparison of Syringin Content Between Cell Cultures and Botanical Drug of Saussurea involucrata[J]. Chinese Pharmaceutical Journal, 2008, 43(10): 737-738
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