OBJECTIVE To research the optimal process of ultrahigh pressure extraction(UPE) of Saponin from Radix Astragali.METHODS The contents of astragaloside IV were taken as the indices and were determined by a HPLC equipped with ELSD detector.The extraction pressures(100,300,500 MPa),concentrations of ethanol(50%,70% and 90%),ratio of solvent to material(10∶1,15∶1 and 20∶1) and extraction time(1,3 and 5 min) were analyzed by orthogonal design,the best UPE condition was ascertained and compared with hot reflux extraction.RESULTS Ultrahigh pressure with 300 MPa,70% methanol,20 of the ratio of solvent to material(mL·g-1) and 5 min extraction time were the optimized condition for the extraction of astragaloside IV from Radix Astragali.The efficiency of UPE was better than heat reflux extraction.CONCLUSION Compared with heat reflux extraction methods,the UPE of astragaloside IV from Radix Astragali is efficient,less extraction time,lower energy consumption and so on.
Key words
Superhigh pressure /
extraction /
radix astragali /
astragaloside IV
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