Departement of Pharmacy，Hainan Medical College，Hainan Provincial Key Laboratory of R＆D of Tropical Herbs，Haikou 571101，China
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Received
Published
2007-05-31
2008-02-05
Issue Date
2008-02-05
Abstract
OBJECTIVE To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS).METHODS The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected.Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl.After acid hydrolysis,the analytes of interest were separated on a SUPELCOSILTMC18 column.The mobile phase was methonl-acetonitrile-1% acetic acid(20:30:50)with a flow rate of 0.8 mL·min-1.A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray(EPI) source was used as detector and was operated in negative ion mode.RESULTS Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry,i.e.m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin.CONCLUSION Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma.And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.
CHEN Feng;FU Ni-gung;REN Shou-zhong;WEI N;ZHNG Jun-qing.
Determination of Quercetin Metabolites in Rat Plasma by Liquid Chromatography-Mass Spectrometry [J]. Chinese Pharmaceutical Journal, 2008, 43(03): 225-227
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