New Mechanism of Jurkat T Cells Apoptosis Induced by Vincristine Via Inhibiting the Expression of SODD Protein
TAO Hong-fang1，HU Qun1*，FANG Jian-lin2，LIU Ai-guo1，LIU Shuang-you1，ZHANG Liu-qing1，HU Ying1
Author information+
1.Department of Pediatrics，Tong-ji Hospital，Huazhong University of Science and Technology Wuhan 430030，China；2.Departmant of Radiology，Union Hospital, Huazhong University of Science and Technology Wuhan 430030，China
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History+
Received
Published
2006-12-25
2008-02-05
Issue Date
2008-02-05
Abstract
OBJECTIVE To study the changes of silencer of death domains(SODD),caspase3,caspase8 and caspase9 during the apoptotic process of human acute lymphoblastic leukemia Jurkat T cells induced by VCR and explore the new mechanism of tumor cell apoptosis induced by VCR.METHODS After Jurkat cells were induced by VCR,translocated phosphatidylserine was labeled with Annexin V/PI.The apoptosis incidence was measured by FCM.The changes of the expression of SODD,caspase3,caspase8 and caspase9 proteins were determined by western blotting.The secretion of TNF-α was determined by ELISA.The changes of TNFR1 mRNA were determined by RT-PCR.RESULTS Over-expression of SODD in Jurkat cells inhibited the apoptosis of tumor cell,VCR induced the apoptosis of Jurkat cells via down-regulating SODD.caspase3 and caspase8 were hydrolysated in a time-dependent manner,in the process,caspase9 was not been deregulated.While the secretion of TNF-α and the expression of TNFR1 in Jurkat cells were not changed by VCR.CONCLUSION VCR Induces the apoptosis of Jurkat cells via downregulating protein expression of SODD which need not activate the signal pathway of TNF/TNFR1 and starts up extrinsic apoptosis pathway namely cascades including caspase8 and caspase3.
TO Hong-fng;HU Qun;FNG Jin-lin;LIU i-guo;LIU Shung-you;ZHNG Liu-qing;HU Ying.
New Mechanism of Jurkat T Cells Apoptosis Induced by Vincristine Via Inhibiting the Expression of SODD Protein [J]. Chinese Pharmaceutical Journal, 2008, 43(03): 180-183
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