1.302 Hospital of PLA&PLA Institute of Chinese Materia Medica，Beijing 100039，China；2.Key Lab of Modern Preparation of TCM，Ministry of Education，Nanchang 330004，China
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Received
Published
2007-01-03
2008-01-10
Issue Date
2008-01-10
Abstract
OBJECTIVE To prepare puerarin submicron emulsions,and evaluate its anti-hemolysis side effect.METHODS The puerarin submicron emulsion was prepared by the novel complex-phase inversion-high press homogenization technology,and compared with puerarin injection,the protective mechanism of its anti-hemolysis activity was studied in terms of red blood cell values,packed cell volume,plasma hemoglobin level,haptoglobin level and osmotic fragility.RESULTS The mean diameter,Zeta potential and entrapment efficiency of emulsion prepared by this method were 188.14 nm,-29.03 mV and 89.72%,respectively.And the red blood cell values,packed cell volume,plasma hemoglobin level,haptoglobin level and osmotic fragility of puerarin submicron emulsion were significantly different(P<0.05),compared with those of control group.Puerarin submicron emulsion reduced the hemolysis activity of puerarin,but puerarin injection resulted in the hemolysis side effect in vivo.CONCLUSION Puerarin submicron emulsion prepared by complex-phase inversion-high press homogenization technology reduced the hemolysis side effect of puerarin by the incorporation of drug.Submicron emulsion is more safe and effective system for puerarin.
YUE Peng-fei;YUN Hi-long;CONG Long-o;WNG Qi;YNG Ming;ZHU Wei-feng;XIO Xio-he.
Study on Preparation and Anti-Hemolysis Activity of Puerarin Submicron Emulsion [J]. Chinese Pharmaceutical Journal, 2008, 43(02): 119-122
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