Study on Optimization of Preparation and Formulation of Docetaxel-Containing Liposomes

HUNG Hong-ing;LIU To;XU Yue-hong;LIN Zi-cho;ZHONG Jin-tng;LIU Qing;ZHENG Ying-ying;RUN Hui-shi

Chinese Pharmaceutical Journal ›› 2007, Vol. 42 ›› Issue (24) : 1872-1876.

Chinese Pharmaceutical Journal ›› 2007, Vol. 42 ›› Issue (24) : 1872-1876.
论著

Study on Optimization of Preparation and Formulation of Docetaxel-Containing Liposomes

  • HUANG Hong-bing 1，LIU Tao1*，XU Yue-hong2，LIN Zi-chao1，ZHONG Jin-tang1，LIU Qing1，ZHENG Ying-ying2，RUAN Hui-shi2
Author information +
History +

Abstract

OBJECTIVE To optimize the preparation and formulation of docetaxel-containing liposomes.METHODS Orthogonal design was adopted to screen the preparation and formulation of docetaxel-containing liposomes on the basis of the comprehensive score of the encapsulation efficiency,particle size and stability of liposomes.RESULTS The ideal combination of preparation and formulation were as follows: docetaxel-(lecithin+cholesterol)=1∶20;lecithin-cholesterol=6∶1.The formulation was prepared by thin-film ultrasonic dispersion technique using specified volume of PBS as hydrating medium.The concentration of liposome solution was 0.5 g·L-1.The average encapsulation efficiency of the optimized liposome was(84.4±1.6)%,the average particle size was(147.1±41.1)nm and the Zeta potential was(+2.25±0.2)mV.The docetaxel liposome was quite stable after being stored in refrigerator for 6 months.The sustained release was obtained in vitro.CONCLUSION The thin-film ultrasonic method and the optimized preparation can be used to prepare docetaxel liposome successfully.

Key words

docetaxel / liposome / orthogonal design

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
HUNG Hong-ing;LIU To;XU Yue-hong;LIN Zi-cho;ZHONG Jin-tng;LIU Qing;ZHENG Ying-ying;RUN Hui-shi. Study on Optimization of Preparation and Formulation of Docetaxel-Containing Liposomes [J]. Chinese Pharmaceutical Journal, 2007, 42(24): 1872-1876

References

[1] GAO W，JOHNSON J L，KHAN S，et al.Paclitaxel quantification in mouse plasma and tissues containing liposome-entrapped paclitaxel by liquid chromatography-tandem mass spectrometry：application to a pharmacokinetics study[J] .Anal Biochem，2005，336：213-220. [2] MARIA L I，PAOLA B，SILVIA A，et al.Preparation，characterization，cytotoxicity and pharmacokinetics of liposomes containing docetaxel[J] .J Controlled Release，2003，91：417-429. [3] WANG Q W，LIU H，LU H L，et al.Enhancement of radiosensitivity by immunoliposomal docetaxel in human colon adenocarcinoma LoVo cell[J] .Chin J Exp Sur（中华实验外科杂志），2006，23（7）：795-797. [4] ZHANG G F，ZHU J B.Study on optimiaze the preparation and formulation nimodipine-containing nanoliposome[J] .J China Pharm Univ（中国药科大学学报），2003，34（1）：25-28. [5] GAO S，ZHONG Y Q.New Dosage Form and New Technology of Modernistic Drug（现代药物新剂型新技术）[M] .Beijing：People′s Military Medical Publisher，2003：207. [6] ZHU L，WANG L L，NI K Y，et al.Preparation of stable magnetoliposomes with high encapsulation efficiency of methotrexate[J] . Chin Pharm J(中国药学杂志)，2006，41（17）：1324-1327. [7] STEPHEN J C，LAURENT P R.Clinical pharmacokinetics of docetaxel[J] .Chin Pharm J（中国药学杂志），1999，36(2)：99-114.

Accesses

Citation

Detail
Sections
Recommended

/

〈 〉