Abstract
OBJECTIVE To investigate the protective effects and mechanism of sodium ferulate on focal cerebral ischemia/reperfusion injury in rats.METHODS The models of focal brain ischemia/reperfusion injury was prepared by middle cerebral artery occlusion(MCAO)in Wistar rats.One hundred and eight male Wistar rats were randomly divided into six groups:sham group,ischemia/reperfusion injury group,nimodipine group and three sodium ferulate groups,sodium ferulate was administered in the doses of 25,50,100 mg·kg-1,respectively.One hour after ischemia and twenty-four hours after reperfusion,the extent of neurological deficits was evaluated by the method of Longa,the infarction volumes were showed with 2,3,5-triphenyltetrazoliumchloride(TTC) staining and evaluated by image analysis system.The pathological changes were determined by HE staining,and then the levels of ET and CGRP in brain tissue were measured by radioimmunoassay.RESULTS Sodium ferulate significantly ameliorated the neurological deficit and diminished the infarction volume induced by MCAO.And at the same time,sodium ferulate reduced the levels of ET and the ratio of ET/CGRP dose-dependently.CONCLUSION Sodium ferulate can protect the brain from ischemia/reperfusion injury in rats.And the neuroprotective mechanism may be related to down-regulating the levels of ET and ratio of ET/CGRP.
Key words
sodium ferulate /
cerebral ischemia /
reperfusion injury /
endothelin /
calcitonin gene-related peptide
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LIU Ping;WNG Ju-ying;LIU Wen-qin;ZHNG Xio-xi;LI Xio-yn;YNG Gui-zhong;LIU Zho-ping;ZHNG Xiu-mei.
Protective Effects of Sodium Ferulate on Focal Brain Ischemia/Reperfusion Injury by Regulating ET/CGRP of Brain Tissue in Rats [J]. Chinese Pharmaceutical Journal, 2007, 42(24): 1859-1863
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