Determination of Phospholipid and Its Degradation Products in Liposomes for Injection by HPLC-Charged Aerosol Detection(CAD)
JIANG Qing-wei，YANG Rui，MEI Xing-guo
Author information+
Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing 100850，China
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History+
Received
Published
2006-12-11
2007-12-05
Issue Date
2007-12-05
Abstract
OBJECTIVE To develop a HPLC-CAD method for the determination of DSPE-PEG,phosphatidylcholine(DPPC),DSPG,lysophosphatidylcholine(MPPC) in phospholipid bilayer of blank liposomes for injection.METHODS A separation was performed on a C8 column with the mobile phase of methanol-water gradient elution at the flow rate of 1 mL·min-1. RESULTS The average recoveries of DSPE-PEG,DSPG,MPPC and DPPC were 99.2%,98.7%,99.5% and 99.1% and with RSDs of 1.1%,1.5%,0.8% and 1.3% respectively.CONCLUSION HPLC-CAD,a new method for phospholipids analysis,provides high sensitivity and reproducibility.
JING Qing-wei;YNG Rui;MEI Xing-guo.
Determination of Phospholipid and Its Degradation Products in Liposomes for Injection by HPLC-Charged Aerosol Detection(CAD) [J]. Chinese Pharmaceutical Journal, 2007, 42(23): 1794-1796
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References
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