Abstract
OBJECTIVE To evaluate the pharmacodynamic effects of recombinant human interferon α-2b (IFN α-2b) inhalation powder.METHODS 2′,5′-Oligoadenylate synthetase (OAS) was used as a surrogate marker for IFN α-2b induction,and a sensitive and semi-quantitative method was established by use of reverse transcription-polymerase chain reaction (RT -PCR) for the determination of OAS1 and OAS2 forms of OAS mRNA in peripheral blood mononuclear cells .RESULTS OAS1 mRNA concentration was equivalent after IFN α-2b was administrated by injection and inhalation,but the later administration route resulted in a higher OAS2 mRNA level.CONCLUSION IFN α-2b inhalation powder is promising to be a non-invasive systemic delivery route with excellent compliance.
Key words
interferon α-2b /
inhalations powder /
pharmacodynamic effects /
RT-PCR /
2′ /
5′-oligoadenylate synthetase
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JING Rong-go;WNG Li-qing;LIU Heng;WNG Chun-long.
Pharmacodynamic Evaluation of Interferon α-2b Inhalation Powder by RT-PCR [J]. Chinese Pharmaceutical Journal, 2007, 42(22): 1692-1695
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References
[1] CHIEUX V，HOBER D，CHEHADEH W，et al. Alpha interferon，antiviral proteins and their value in clinical medicine[J] . Ann Biol Clin，1999，57 (6)： 659-666.
[2] CHOUDHARY S，GAO J，LEANMAN D W，et al. Interferon action against human parainfluenza virus type 3： involvement of a novel antiviral pathway in the inhibition of transcription[J] . J Virol，2001，75 (10)：4823-4831.
[3] BALAN V，NELSON D R，SULKOWSKI M S，et al. Modulation of interferon-specific gene expression by albumin-interferon-alpha in interferon-alpha-experienced patients with chronic hepatitis C[J] . Antivir Ther，2006，11(7)： 901-908.
[4] LUO S，CASSIDY W，JEFFERS L，et al. Interferon-stimulated gene expression in black and white hepatitis C patients during peginterferon alfa-2a combination therapy[J] . Clin Gastroenterol Hepatol，2005，3(5)：499-506.
[5] DONG B，SILVERMAN R H. 2-5A-dependent Rnase molecules dimerize during activation by 2-5A[J] . J Biol Chem，1995，270 (8)： 4133-4137.
[6] SAWAI H，ISHIBASHI K，ITOH M，et al. Sensitive radioimmuno assay for 2′，5′-oligoadenylates using a novel 125I-labeled derivative of 2′，5′-triadenylate 5′-triphosphate[J] . J Biochem，1985，98 (4)： 999-1005.
[7] FENG Y F，YANG K Y. A rapid non-isotope assay for 2′，5′-oligoadenylate synthetase[J] . Chin Biochem J（生物化学杂志），1997，13 (4)： 428-431.
[8] WILLS R J，DENNIS S，SPIEGEL H E，et al. Interferon kinetics and adverse reactions after intravenous，intramuscular，and subcutaneous injection[J] . Clin Pharmacol Ther，1984，35 (5)：722-777.
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