Abstract
OBJECTIVE To establish a new sensitive method for the simultaneous determination of L-carnitine(LC),Acetyl-L-carnitine(ALC) and Propionyl-Lcarnitine(PLC) in Human Plasma by HPLC.METHODS The analytes were extracted by protein precipitation and then precolumn derivatization with l-aminoanthracene (IAA) was performed. The fluorescent derivatives were separated on a Hypersil C18 column,and the mobile phase consisted of acetonitrile-0.1 mol·L-1 ammonium acetate(28∶72),the flow rate was 1.0 mL·min-1. The derivatives were monitored with a fluorescent detector set at 248 nm (excitation wavelength) and 418 nm (emission wavelength). RESULTS The good linearity was observed over the concentration ranges of 5～160 μmol· L-1 for LC,1～32 μmol· L-1 for ALC,0.25～8 μmol· L-1 for PLC. The recoveries of LC,ALC and PLC were 99.48%,99.94% and 98.20%,respectively. The intra-and inter-day validation were adequate with the RSDs of 5.83% or below.CONCLUSION This method is proved to be stable,accurate and convenient to study the concentrations of LC,ALC and PLC in plasma.
Key words
L-carnitine /
acetyl-L-carnitine /
propionyl-L-carnitine /
HPLC
{{custom_keyword}} /
Cite this article
Download Citations
SUN Yong-xu;LU Cong-xio;TNG Qi-ling;CO Yu;XUE Yu-zeng;WN Xiu-xi;WNG Chun-o.
Simultaneous Analysis of L-carnitine,Acetyl-L-carnitine and Propionyl-L-carnitine in Human Plasma by HPLC [J]. Chinese Pharmaceutical Journal, 2007, 42(18): 1425-1428
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] ANGSTEN G，CEDERBLAD G，MEURLING S. Reference ranges for muscle carnitine concentration in children[J] .Ann Clin Biochem，2003，40(4)：404-410.
[2] LIN F.The application of L-carnitine in holo-parenteral alimentation[J] .Chin J Gastrointestinal Surgery(中华胃肠道杂志)，2003，3(1)： 42.
[3] ANGELINI C，VERGANI L，MARTINUZZI A. Clinical and biochemical aspects of carnitine deficiency and insufficiency： transport defects and inborn errors of oxidation[J] . Crit Rev Clin Lab Sci，1992，29 (3-4)： 217-242.
[4] MARZO A，MARTELLI EA，MANCINELLI A，et al. Protein Binding of tine in L-Carnitine Family Components[M] . Participants Papers(Special Issue No III). Eur J Drug Metab Pharmacokinet，1991，364-368.
[5] PACE S，LONGO A，TOON S，et al. Pharmacokinetics of propionyl-L-carnitine in humans： evidence for saturable tubular reabsorption[J] . Br J Clin Pharmacol，2000，50(5)： 441-448.
[6] HOPPEL C. The role of carnitine in normal and altered fatty acid metabolism[J] . Am J Kidney Dis，2003，41(4)： 4 -12.
[7] A LONGO，G. BRUNO，S. CURTI，et al. Determination of L-carnitine，acetyl-L-carnitine andpropionyl-Lcarnitine in human plasma by high-performance liquid chromatography after pre-column derivatization with 1-aminoanthracene[J] . J Chromatography B，1996，686： 129-139.
[8] LAURENCE VERNEZ，MICHAEL DICKENMANN，JURG STEIGER，et al. Effect of L-carnitine on the kinetics of carnitine，acylcarnitines and butyrobetaine in long-term haemodialysis[J] . Nephrol Dial Transplant，2006，21： 450-458.
[9] DING F，GU Y，LIN S T，et al.The multicentre trabant study of curing carntine deficiency by giving levocarnitine[J] . Shanghai Med J（上海医学），2003，26(11)： 785-788.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
