Simultaneous Analysis of L-carnitine,Acetyl-L-carnitine and Propionyl-L-carnitine in Human Plasma by HPLC
SUN Yong-xu1，LU Cong-xiao1，TANG Qi-ling1，CAO Yu2，XUE Yu-zeng3，WAN Xiu-xia2，WANG Chun-bo2
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1.Department of Pharmacy，Yantai Yuhuangding Hospital，Affiliated Hospital of Medical College，Qingdao University，Yantai 26400，China；2. Medical College，Qingdao University，Qingdao 266071，China; 3. Liaocheng Hospital，Liaocheng 252000，China
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Received
Published
2007-03-07
2007-09-10
Issue Date
2007-09-10
Abstract
OBJECTIVE To establish a new sensitive method for the simultaneous determination of L-carnitine(LC),Acetyl-L-carnitine(ALC) and Propionyl-Lcarnitine(PLC) in Human Plasma by HPLC.METHODS The analytes were extracted by protein precipitation and then precolumn derivatization with l-aminoanthracene (IAA) was performed. The fluorescent derivatives were separated on a Hypersil C18 column,and the mobile phase consisted of acetonitrile-0.1 mol·L-1 ammonium acetate(28∶72),the flow rate was 1.0 mL·min-1. The derivatives were monitored with a fluorescent detector set at 248 nm (excitation wavelength) and 418 nm (emission wavelength). RESULTS The good linearity was observed over the concentration ranges of 5～160 μmol· L-1 for LC,1～32 μmol· L-1 for ALC,0.25～8 μmol· L-1 for PLC. The recoveries of LC,ALC and PLC were 99.48%,99.94% and 98.20%,respectively. The intra-and inter-day validation were adequate with the RSDs of 5.83% or below.CONCLUSION This method is proved to be stable,accurate and convenient to study the concentrations of LC,ALC and PLC in plasma.
SUN Yong-xu;LU Cong-xio;TNG Qi-ling;CO Yu;XUE Yu-zeng;WN Xiu-xi;WNG Chun-o.
Simultaneous Analysis of L-carnitine,Acetyl-L-carnitine and Propionyl-L-carnitine in Human Plasma by HPLC [J]. Chinese Pharmaceutical Journal, 2007, 42(18): 1425-1428
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