Determination of Biotin,Folic acid and Cyanocobalamin in 13 Vitamins for Injection by High Performance Liquid Chromatography
SONG Jin-chun1，LU Xun2，ZENG Jun-fen1，HUANG Ling2
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1.Department of Pharmacy，Renmin Hospital of Wuhan University，Wuhan 430060，China；2.College of Pharmacy，Wuhan University，Wuhan 430072，China
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Received
Published
2006-08-03
2007-09-05
Issue Date
2007-09-05
Abstract
OBJECTIVE To establish a HPLC method for the simultaneous determination of biotin,folic acid and vitamin cyanocobalamin in water-soluble vitamin for Intersil.METHODS An Intersil(ODS-2)C18 column(4.6 mm×150 mm,5 μm)was used as separation column at 30 ℃.The mobile phase consisted of 0.05 mol·L-1 KH2PO4 solution(adjusted to pH 3.0 with phosphoric acid)-acetonitrile(90∶10).The flow rate was 1.0 mL·min-1.The detection wavelength was at 210 nm.The injection volume was 10 μL.RESULTS The detection limits of biotin,folic acid and cyanocobalamin were 0.10,0.11 and 0.82 μg·L-1,respectively.The linear ranges of biotin,folic acid and cyanocobalamin were in the ranges of 9.49～14.23,96.74～145.12 and 0.746～1.118 mg·L-1,respectively.The average recoveries were about 101.00%.CONCLUSION The method was simple,rapid,sensitive and reproducible.
SONG Jin-chun;LU Xun;ZENG Jun-fen;HUNG Ling.
Determination of Biotin,Folic acid and Cyanocobalamin in 13 Vitamins for Injection by High Performance Liquid Chromatography [J]. Chinese Pharmaceutical Journal, 2007, 42(17): 1345-1347
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References
[1] YU L L，HUANG X L，WU H Q. Determination of biotin in fodder by HPLC[J] .J Intrum Anal(分析测试学报)，2003，22（5）：102-104.
[2] REN Y P，ZHANG A Z，ZHU L Y，et al. Measurement of folic acid in food using HPLC[J] .Food Ferment Ind(食品与发酵工业)，2002，19（10）：46-49.
[3] LONG C Y，GAO Y H，XU X M，et al.Simultaneous determination of vitamin B1 and folic acid by high performance liquid chromatography with post-column chemical derivation and fluorescence detection[J] .Anal Chem(分析化学)，2004，32（10）：1341-1344.
[4] DENG F L，ZHOU P，CHEN B M，et al. Determination of biotin and folic acid with methyl-p-hydroxybenzoate in water-soluble vitamin for injection by high performance liquid chromatography[J] . China New Med(中国新医药)，2004，3（8）：46-48.
[5] YANG Y，LI B，SHI X H. Determination of vitamin B12 in vitamin B12 compound ointment-I by HPLC[J] .Chin J Pharm(中国医药工业杂志)，2004，35(16) ：617-618.
[6] SUI Y R，ZHANG L，HUANG Z D，et al. Studies on determination of vitamin B6，vitamin B12 and folic acid in compound folic acid tablets by ion pair chromatography[J] .China J Pharm Anal(药物分析杂志)，2003(23)，4：291-292.
[7] LI H B，CHEN F. Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection[J] .Chromatographia，2001，54：270-273.
[8] LI H B，CHEN F. Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection [J] .Sep Sci，2001，24：271-274.
[9] HEUDI O，KILINC T，FONTANNAZ P. Separation of water-soluble vitamins by reversed-phase high performance liquid chromatography with ultra-violet detection： Application to polyvitaminated premixes[J] .J Chromatography A，1070 (2005)： 49-56.
[10] OLIVIER H，TAMARA K，PATRIC F.Separation of water-soluble vitamins by reversed-phase high performance liquid chromatography with ultra-violet detection：Application to polyvitaminated premixes[J] .Chromatography A，2005，（1070）：49-56.
