OBJECTIVE To establish chiral HPLC methods for the enantiomeric separation of fourteen drugs,such as flurbiprofen and bambuterol.METHODS Enantiomeric separation of fourteen compounds was performed on a chiralpak AD column.The chiral separation of eight acid compounds(flurbiprofen,ketoprofen,mandelic acid,fluvastatin,o-chloro mandelic acid,etodolac,ibuprofen and naproxen)and six basic compounds(bambuterol,propafenone,cetirizine,nitrendipine,felodipine and nimodipine)was investigated with the mobile phase of consisting n-hexane,ethanol and different proporation of trifluoroacetic acid(TFA)or triethylamine(TEA).The mobile phase compositions were systematically investigated to obtain the optimal chromatographic profile.RESULTS The ratio of n-hexane/ethanol and the contents of acid or basic additive were the primary influencing factors for chiral separation.The good resolutions of acidic compounds flurbiprofen,ketoprofen,mandelic acid,fluvastatin,o-chloro mandelic acid,etodolac and ibuprofen were obtained with the resolution factors of 11.0,12.5,4.70,5.30,1.90,1.50 and 1.60 respectively,while the content of n-hexane was 90%～99% and TFA content was 0.1%～0.3%.The separation of enantiomers of naproxen wasn't obtained.After gradually enhanced the ratio of n-hexane and the content of TEA in mobile phase of n-hexane-ethanol-TEA,the baseline enantioseparation was obtained for basic compounds propafenone,cetirizine,bambuterol,nitrendipine and felodipine,and their resolution was 8.20,3.0,1.90,1.90 and 1.60 respectively,but nimodipine couldn't be separated.CONCLUSION Twelve compounds are separated successfully on the chiralpak AD column under suitable chromatographic conditions.