Abstract
OBJECTIVE To investigate the protective effects of oxymatrine on myocyte apoptosis induced by acute myocardial ischaemia in rats and to explore its possible mechanism.METHODS Rat myocardial ischaemia model was induced by ligating the anterior descending branch of left coronary artery.At six hours after ischaemia,the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)were determined to confirm the degree of injury,histopathological changes in myocardium was observed,apoptotic cells were detected using the terminal deoxynucleotidyl transferase-mediated d-UTP nick end labeling(TUNEL)methods,the expressions of Fas/FasL and Bcl-2 were studied by SP immunohistochemical method.RESULTS Oxymatrine reduced the size of myocardial infarct,increased SOD activity and reduced MDA products in serum.Oxymatrine reduced the pathological injury of myocardium,decreased apoptosis index and the myocardial expression of Fas protein,increased the expression of Bcl-2 protein,whereas the expression of FasL protein was not affected.CONCLUSION Oxymatrine has protective effects on acute myocardial ischaemia in rats.These effects may be related to decreasing oxygen free radical,decreasing the myocardial expression of Fas protein and increasing the expression of Bcl-2 protein.
Key words
oxymatrine /
myocardial ischaemia /
cell apoptosis
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SHNG Lei;WNG Ling;SUN Hong-li;CHU Wen-feng;YNG o-feng.
Protective Effects of Oxymatrine on Myocyte Apoptosis in Rats with Acute Myocardial Ischaemia [J]. Chinese Pharmaceutical Journal, 2007, 42(07): 501-504
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