Protective Effects of Breviscapine on Learning-Memory Dysfunction Induced by Ischemia-Reperfusion in Mice
YIN Ming-hua， X Xiao-hong*， JIANG Ke-sheng
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College of Chemistry and Life Science， Zhejiang Normal University， Jinhua 321004， China
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Received
Published
2006-02-05
2007-04-05
Issue Date
2007-04-05
Abstract
OBJECTIVE To study the protective effects of breviscapine on learning-memory dysfunction induced by cerebral ischemia-reperfusion in mice.METHODS The mice were divided randomly into sham group,model group,positive control group(extract of leaves of Ginkgo biloba L.,Gb,35 mg·kg-1)and breviscapine treated group(Bre,80,20,5 mg·kg-1).After accommodated to new circumstances for 3 d,the mice of positive control and Bre treated groups were treated with Gb of 35 mg·kg-1 and Bre of 80,20,5 mg·kg-1(ip)respectively once daily for 7 d,while sham and model groups were injected with 0.9% NaCl(ip).At thirty minutes after drug treatment on day 7,the mice were anesthetized(ethyl carbamate,1.275 g·kg-1,ip),and ischemia-reperfusion operations were conducted.Repeated cerebral ischemia/reperfusion was induced by fastening bilateral common carotid artery.The next day following surgery,the spontaneous behavior was tested using open field.And learning-memory was measured using Morris water maze on day 3 after surgery.The activity of SOD and the content of MDA were then measured by UV-photospectrometer analysis system.Meanwhile,the inhibiton of breviscapine on oxidative hemolysis induced by H2O2in vitro was detected.RESULTS Ischemia-reperfusion did not affect the spontaneous behavior,but impaired learning-memory,declined the activity of SOD,and increased the level of MDA in mice.It was found that breviscapine(5,20,80 mg·kg-1)and Gb remarkably improved learning-memory ability(P<0.05,P<0.01),meanwhile promoted the activity of SOD(P<0.01)and decreased the content of MDA(P<0.05,P<0.01)when compared to the ischemic group.The experiment in vitro showed breviscapine(0.015～1.5 g·L-1)dose-dependently decreased oxidative hemolysis(P<0.05,P<0.01).CONCLUSION Breviscapine shows an improvement effect against the memory impairment in mice induced by ischemia/reperfusion.The improvement of the anti-oxidation activity in brain may be involved in.
YIN Ming-hu;X Xio-hong;JING Ke-sheng.
Protective Effects of Breviscapine on Learning-Memory Dysfunction Induced by Ischemia-Reperfusion in Mice [J]. Chinese Pharmaceutical Journal, 2007, 42(07): 497-500
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