Abstract
OBJECTIVE To establish a quantitative RP-HPLC method for the simultaneous determination of alantolactone and isoalantolactone in herb Inula racemosa Hook.f..METHODS A column of Phenomenex Kromasil C18(4.6 mm×250 mm,5.0 μm)was used.The mobile phase consisted of acetonitrile and 0.04% phosphate(50∶50).The flow rate was 1.0 mL·min-1 with UV detection wavelength at 194 nm.RESULTS Isomer alantolactone and isoalantolactone showed good separable degree.A good linearity was obtained with the correlation coefficients ranged of 0.07～4.80 μg·L-1(r=0.999 8)and 0.07～4.85μg·L-1(r=0.999 8).The average recoveries were 97.5% and 102.1%.The precision and accuracy of the assay were 1.56%and 1.87%(n=5).CONCLUSION The method is rapid,accurate,reliable and can be used to control the quality of herb Inula racemosa Hook.f.and it products.
Key words
RP-HPLC /
Inula racemosa Hook.f. /
alantolactone /
isoalantolactone /
determination
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XIO Yun-cn;HU Feng-zu.
Determination of Alantolactone and Isoalantolactone in Herb Inula racemosa Hook.f.by RP-HPLC [J]. Chinese Pharmaceutical Journal, 2007, 42(07): 491-493
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