OBJECTIVE To study the chemical constituents of Streptocaulon griffithii.METHODS Various column chromatographic techniques were used for the isolation and purification.The structures were elucidated on the basis of spectral data and chemical evidences.RESULTS Seven cardenolides were obtained and identified as digitoxogenin(Ⅰ),16-O-acetylgitoxigenin(Ⅱ),periplogenin(Ⅲ),16-O-acetylperiplogenin(Ⅳ),periplogenin digitoxoside(Ⅴ),periplogenin-3-O-β-D-glucopyranosyl-(1→4)-O-β-D-digitoxopryanoside(Ⅵ),periplogenin-glucoside(Ⅶ).CONCLUSION All the compounds are obtained from Streptocaulon griffithii for the first time and compound Ⅳ is isolated for the first time in nature.