Abstract
OBJECTIVE To investigate the effect of selenuim(Se) on the expression of immune molecules and multiple gene in peripheral blood mononuclear cells from the allogeneic heart transplantation rats. METHODS Rats were divided into control group, Se and CsA experimental group and orally treated with water, Se (40 μg·kg-1) and CsA (15 mg·kg-1) respectively. Mononuclear cells from peripheral blood were collected at 1, 3, 7, 10 and 12 d post-transplantation. Mono-clone antibodies were used to label surface molecules of the cells. The expression of MHC-Ⅱ, CD4, CD8 IL-2R and ICAM-Ⅰ was separately evaluated by flow-cytometer.The expression of multiple gene was evaluated by DD-PCR. Myocardial pathological changes were classified in according to the international standard. RESULTS In control group,the surrival rate of graft heart was reduced at 3th day after transplantation and rapid reduced at 7～12 d. In Se grorp,the surrival rate of graft heart was reduced at 7th day and there was 43% surrival rate of graft heart at 10～12 d.In CsA group,the surrival rate of graft heart was reduced by 50% at the 3th day after transplantation. There was 30% survival rate of graft heart at post-transplantation 12 d.②The pathological damages of graft heart characterized with Ⅱ phase were 12.5% and 33.3% in control group and CsA group respectively after 24 h post-transplantation. In Se group,Ⅱ phase pathological alteration occured after 7 day post-transplantation.③Compared with control group, the immune molecules of MHC-Ⅱ,CD8,IL-2R and ICAM-Ⅰ were significantly down-regulated in Se group(P<0.05 or P<0.01).Immune molecule IL-2R was significantly down-regulated in CsA group.④Compared with control group, Se and CsA inhibited the expression of 2,3-bisphosphoglycerate. In Se group, the expression of gig18 was significantly down-regulated but up-regulated in CsA group.CONCLUSION Se increases the surrival rate of graft heart and protects the graft heart by up and down-regulating genes transfer of 2,3-bisphosphoglycerate and gig18 which relevant to cell metabolism and apoptosis and by inhibiting the expression of MHC-Ⅱ,CD8,IL-2R and ICAM-Ⅰ.
Key words
rat /
heart transplantation /
selenium /
immune molecule /
gene /
expression
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GU Yun;WNG Sheng-yu;HUNG Yi-min;ZHNG Yong-ke;Lü Yn-ning;ZHNG Ying;GE Liu-ping.
Effect of Selenium on Expression of Immune Molecules and Multiple Gene in Peripheral Blood Mononuclear Cells from Allogeneic Heart Transplantation Rats [J]. Chinese Pharmaceutical Journal, 2006, 41(24): 1866-1871
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