Studies on Noncovalent Binding Between Puerarin and Plasma Protein by Electrospray Ion Trap Mass Spectrometry
QI Chun-hua，HAN Feng-mei，CAO Yi，CHEN Yong
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Hubei Province Key Lab of Bio-Technology of TCM，Hubei University，Wuhan 430062，China
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Received
Published
2005-12-10
2006-12-05
Issue Date
2006-12-05
Abstract
OBJECTIVE To study the noncovalent binding between puerarin (PU) and two plasma proteins,such as human serum albumin(HSA) and α1 -acid glycoprotein(AAG). METHODS The molecular weight of puerarin, human serum albumin,α1-acid glycoprotein and their complexes were measured by the electrospray iontrap mass spectrometry(ESI-MS). The maximum stoichiometric ratios were obtained according to the molecular weight change of the complexes before and after binding reaction, the binding constants of the complexes were calculated by the scatchard equation. The main sorts of binding force of the complexes were deduced according to the relationship between the reaction temperature and the thermodynamic parameters . RESULTS The binding constants(K) and the maximum stoichiometric ratios were 7.59×104mol·L-1,and 1∶5 for PU-HSA,and 8.01×104 mol·L-1 and 1∶8 for PU-AAG, respectively.The main sorts of binding force of the complexes between puerarin and the two plasma proteins were static-electricity gravitation. CONCLUSION ESI-MS is a good method for studying the drug-protein noncovalent binding due to its some advantages in sensitivity, high-speed and accuracy .
QI Chun-hu;HN Feng-mei;CO Yi;CHEN Yong.
Studies on Noncovalent Binding Between Puerarin and Plasma Protein by Electrospray Ion Trap Mass Spectrometry [J]. Chinese Pharmaceutical Journal, 2006, 41(23): 1822-1825
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