Abstract
OBJECTIVE To establish a RP-HPLC method for determinating palonosetron in human liver microsomes. METHODS Palonosetron in human liver microsomal incubates was extracted by diethyl ether and diphenytriazol was used as internal standard. The determination was performed on a Diamonsil C18 column(4.6 mm×200 mm, 5 μm) with a mobile phase consisting of 0.01 mol·L-1 KH2 PO4 (pH 3.0)and methanol (30∶70) at a flow-rate of 1.0 mL·min-1.UV detector was at 241 nm.RESULTS The assay was linear over the range of 1～100 μmol·L-1 for palonosetron (r=0.999 9).The limit of detection was 0.05 μmol·L-1(S/N≥3) and the limit of quantification was (0.21±0.04) μmol·L-1(n=5).The assay recovery and the absolute recovery were 89.7% and 100.0%, respectively. The inter-day and intra-day variation coefficients were all less than 10% (n=5).CONCLUSION The method developed is applied successfully to study the metabolism of palonosetron in human liver microsomes.
Key words
palonosetron /
HPLC
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ZHO N-ping;YU Lu-shn;YO Tong-wei;ZENG Su.
Determination of Palonosetron in Human Liver Microsomes by High-Performance Liquid Chromatography with UV-Detection [J]. Chinese Pharmaceutical Journal, 2006, 41(21): 1654-1656
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