1.Department of Pharm-analysis，College of Pharmacy，Fudan University，Shanghai 200032，China；2.Department of Eye & ENT Hospital, Fudan University, Shanghai 200031, China
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History+
Received
Published
2005-08-12
2006-07-05
Issue Date
2006-07-05
Abstract
OBJECTIVE To determinate of captopril and hydrochlorothiazide by high performance liquid chromatography.METHODS The HPLC separation was carried out on a Diamonsil C18(4.6 mm×150 mm,5 μm) column,using a UV detector and the wavelength was 263 nm.Elution was carried out with acetate acid solution(solvent A,pH 1.8) and acetonitrile(solvent B) at a flowrate of 1.2 mL·min-1.Gradient HPLC was used with thesolvent ratio changed from 90∶10(for 5 min),to 60∶40(after 8 min) for solvent A∶B,respectively.The internal standard was sulfadiazine.RESULTS The peak area ratios of captopril or hydrochlorothiazide to internal standard were linear within a certain range.The extraction recoveries were more than 70%.The relative standard derivations of within-day and between-day were less than 15%.CONCLUSION The method is proved to be accurate,sensitive and reliable.It is suitable for the determination in human plasma of the new combination formulation of captopril and hydrochlorothiazide.
HUNG To-min;HE Zhong;ZHENG Xio-wei;SHO Lu-ping;DUN Geng-li.
Determination of Captopril and Hydrochlorothiazide in Serum by High Performance Liquid Chromatography [J]. Chinese Pharmaceutical Journal, 2006, 41(13): 1010-1012
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References
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