Abstract
OBJECTIVE To study the synthesis,purification,analysis and activity determination of Thymus Humoral Factor(THF-γ2).METHODS Thymus Humoral Factor(THF-γ2) was synthesized by FmocSPPS,purified by MPLC,and identified by HPLC,MS and amino acid sequence determination.The biological activity of THF-γ2 was measured according to its characteristics of promoting IL-2 production in mouse spleen cells. RESULTS The purity of the product was 95.92%,the molecular mass was 918,and the amino acid sequence was NH2/Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu.In the concentration of 300 μg·L-1,the activity was largest and 1.9 times as much as the control.CONCLUSION The methods of the synthesis,purification,analysis and activity determination of Thymus Humoral Factor(THF-γ2) are established for further research and applications in China.
Key words
thymus humoral factor /
Fmoc /
IL-2
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LIO Xio-qun;ZHO Zong-ge;ZHOU Li-n;XU Kng-sen;YUN Qin-sheng.
Synthesis and Activity Determination of Thymus Humoral Factor(THF-γ2) [J]. Chinese Pharmaceutical Journal, 2006, 41(07): 548-550
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