Up-regulation of ERβ in Murine Osteoblasts with the Action of Dehydroepiandrosterone
WANG Yu-dong1， TONG Jian-qian1， LUO Lai-min1， LI Da-jin2
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1.Department of Obstetrics and Gynaecology， Sixth People Hospital， Jiaotong University， Shanghai 200233， China； 2.Department of Reproductive Immunology， Laboratory of Obstetrics& Gynaecology， Fudan University， Shanghai 200011， China
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History+
Received
Published
2005-04-20
2006-04-05
Issue Date
2006-04-05
Abstract
OBJECTIVE To investigate the up-regulation of ERβ in osteoblast and the mechanism for preventing and curing postmenopausal osteoporosis with dehydroepiandrosterone(DHEA).METHODS Murine osteoblasts(OBs) from calvaria of neonatal BALB/c mice were cultured and treated with 1×10-7 mol·L-1 DHEA at different time after the estrogen withdrawal.The biological characteristics of OBs were evaluated by transmission electroscope.The mRNA level of osteoprotegerin(OPG)/receptor activator of NF-κB ligand(RANKL) and estrogen receptor(ER) isoforms were detected by RT-PCR.The correlation between ER isoform and OPG/RANKL was analyzed,too.RESULTS With the action of 1×10-7 mol·L-1 DHEA.The OBs had more organelles and less glycogen.The expression of OPG/RANKL mRNA(P<0.01) and ERβ mRNA(P<0.01) in OBs was improved and the ratio of OPG/RANKL mRNA was positively related to the expression of ERβ mRNA(P<0.01).No significance was obtained in the expression of ERα.CONCLUSION DHEA can improve the expression of OPG/RANKL so as to inhibit the bone resorption of OC,which is mediated by the up-regulation of ERβ in OBs.
WNG Yu-dong;TONG Jin-qin;LUO Li-min;LI D-jin.
Up-regulation of ERβ in Murine Osteoblasts with the Action of Dehydroepiandrosterone [J]. Chinese Pharmaceutical Journal, 2006, 41(07): 509-511
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