OBJECTIVE To study the pharmacokinetics and relative bioavailability of tropisetron hydrochloride capsules in healthy volunteers and to evaluate the bioequivalence.METHODS A single dose of 10 mg reference and test tropisetron capsules was given to 18 healthy volunteers in a randomized 2-way cross-over study.The concentrations of tropisetron hydrochloride in plasma were determined by RP-HPLC-UV.RESULTS The main pharmacokinetic parameters of two formulations,reference and test drug were as follows:t_max(1.86±0.43) and(2.03±0.52)h,ρ_max(11.73±3.05) and(11.25±3.30) μg·L^-1,AUC_0～24(111.89±39.86) and(113.61±40.34) μg·h·L^-1;AUC_0-inf(139.71±60.35) and(142.22±57.34) μg·h·L^-1,t_1/2(kα)(0.79±0.29) and(0.82±0.25)h;t_1/2(ke)(5.08±1.92) and((5.48±2.08)) h,Ke(0.16±0.06) and(0.14±0.05) h^-1,respectively.The relative bioavailability of AUC_0～24 and AUC_0-inf were(98.55±10.37)% and(99.77±18.66)%.Using 3P97 program,the results of variance and two one-sided test statistical analysis for Lnρ_max,LnAUC_0～24 and LnAUC_0-inf showed that two formulations were bioequivalent.CONCLUSION The reference and test tropisetron capsules are bioequivalent.