Abstract
OBJECTIVE To establish the digital fingerprint spectrum(FPS)of tea polyphenols, combining with the determination of major components, as a new mode to evaluate and control the quality of tea polyphenols.METHODS The separation was performed on a C18 column with a methanol-water-0.1% formic acid gradient elution system, the detection wavelength was at 210 nm.RESULTS Thirty-three batches of tea polyphenols samples were analyzed by the HPLC-DAD chromatographic system and FPS was established according to the obtained data. The analytical results of methodologic experiments were favorable. CONCLUSION The FPS of tea polyphenols is accurate and feasible, it reflects the real constitution and inherent quality of samples. The FPS of tea polyphenols is suitable for the quality control. The detection ratio of characteristic peaks in tea polyphenols samples should be above 80%, and the relative area (magnified one hundred times) of caffeine should be lower than five.
Key words
tea polyphenols /
chromatographic FPS /
quality control
{{custom_keyword}} /
Cite this article
Download Citations
HE Yu;HONG Xio-kun;WNG Zhi-hu.
Study on HPLC-Fingerprint Quality Control of Thirty-Three Batches of Tea Polyphenols [J]. Chinese Pharmaceutical Journal, 2006, 41(02): 139-142
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] KATIYAR S K，ELMETS C A. Green tea polyphenolic antioxidants and skin photoprotection[J] . Int J Oncol，2001，18(6)：1307-1313.
[2] YANG C S，PRABBU S，LANDAU J. Prevention of carcinogenesis by tea polyphenols[J] . Drug Metab Rev，2001，33(3-4)：237-253.
[3] GONG Y G，YANG X Q，ZHOU S H. Reviews on the high effects of TP scavenging biological free radicals[J] . Nat Prod Res Dev（天然产物研究与开发），1999，11(2)：82-86.
[4] GONG C S. Extraction and application of tea polyphenol[J] . Mod Chem Ind（现代化工），1999，19(3)：14-16.
[5] WEI Y，DING M Y. Chromatographic analysis of tea polyphenols[J] . Chin J Chromatogr（色谱），2000，18(1)：35-38.
[6] DAI J，WANG H X，CHEN W S，et al. Study on the analytical on the catechins in tea and green tea polyphenol samples by high performance liquid chromatography[J] . Chin J Chromatogr（色谱），2001，19(5)：398-402.
[7] BEECHER G R，WARDEN B A，MERKEN H. Analysis of tea polyphenols[J] . Proc Soc Exp Biol Med，1999，220(4)：267-270.
[8] HONG X K，WANG Z H. Digital Chromatographic Fingerprint of Traditional Chinese Medicine（中药数字化色谱指纹谱）[M] . Shanghai： Science and Technology Press，2003.
[9] HE Y，HONG X K，WANG Z H. Determination of catechins and caffeine in tea polyphenols[J] . Chin Tradit Pat Med（中成药），2003，25(10)：827-830.
[10] MENG Q H，LIU Y S，WANG J S，et al. A new algorithm and application of similarity between chromatographic fingerprints[J] . Chin Tradit Pat Med（中成药），2003，25(1)：4-8.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
