Abstract
OBJECTIVE To study the effect of Radix Astragali (RA) on the expression of signal transducer and activator of transcription6 (STAT6) and its mRNA in the bronchus of a rat asthma model.METHODS Forty male SD rats were randomly divided into four groups: the control group,asthma group, AM group treated with high dose,and low dose, respectively. In the experiment, the rat asthma model was established by the ovalbumin(OVA) challenge methods. The lung tissue was gained from the left lung, bronchoalveolar lavage fluid (BALF) was gained from the right lung. The Eosinophils(EOS) numbers and differentiated cell numbers in BALF were counted; the protein expressions of STAT6 were detected by immunohistochemistry techniques; the mRNA expressions of STAT6 were detected by hybridization in situ. RESULTS The total cell numbers in BALF, the absolute numbers of EOS, the ratios of eosinophils to the total cell numbers (EOS%) of asthma group were all significantly higher than those of the control group(P<0.01); The total cell numbers in BALF, the absolute numbers of EOS and EOS% of RA groups were all significantly lower than those of asthma group (P<0.01) . Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group was significantly higher than that of the control group (P<0.01) , while that of RA groups were significantly lower than that of asthma group, the epithelial cells were the chief expression cells; hybridization in situ showed that the mRNA expression of STAT6 around the bronchus of asthma group was significantly higher than that of the control group (P<0.01), while that of RA groups was significantly lower than that of asthma group, the epithelial cells were the chief expression cells. There was a significant correlation between the absolute numbers of EOS in BALF, the content of STAT6 and STAT6mRNA respectively in the epithelial cells of bronchus(P<0.01) .CONCLUSION RA had an inhibitory effect on airway inflammation cells infiltration such as EOS, it significantly depressed STAT6 and its mRNA expression may be key in the modulating mechanism of asthma.
Key words
asthma /
signal transducer and activator of transcription 6 /
Radix Astragali /
epithelial cells
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LI Chng-chong;YE Le-ping;YU Xi-chong;LI Meng-rong;LUO Yun-chun.
Effect of Radix Astragali on Signal Transducer and Activator of Transcription 6 and Its mRNA Expression in a Rat Asthma Model [J]. Chinese Pharmaceutical Journal, 2006, 41(02): 104-107
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(收稿日期：2005-03-21)
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