Purification and its thrombolytic and hematolytic effect of nattokinase in vitro
WANG Ping1，2，CHEN Jun2，CHEN Hong-bin2
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1. College of Annimal Science And Tecnology，Jiangxi Agricultural University，Nanchang 330045，China；2.Department of Pharmacy， School of Biological and Enviromental Engineering， Jiangsu University， Zhenjiang 212013，China
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History+
Received
Published
2005-03-08
2005-11-05
Issue Date
2005-11-05
Abstract
OBJECTIVE To study the thrombolytic and hematolytic effect of crude nattokinase in vitro. METHODS Thrombolytic and hemolytic experiment in vitro were conducted. RESULTS After 6h treatment with crude nattokinase at high dose (2836U) and low dose (1418U), respectively in vitro, 88% and 74% of thrombi were dissolved, respectively, and that of lumbrukinase was 87%. Crude nattokinase haemolyzed rabbit blood red cell. CONCLUSION Crude nattokinase owns thrombolytic and hematolytic function.
WNG Ping;CHEN Jun;CHEN Hong-in.
Purification and its thrombolytic and hematolytic effect of nattokinase in vitro[J]. Chinese Pharmaceutical Journal, 2005, 40(21): 1669-1671
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References
[1］Sumi H. A noval fibrinolytic enzyme in the vegetable cheese natto： a typical and popular soybean food in the Japanese diet[J］. Experientia，1987，43(20)：1110.
[2］Fujita M，Hong K，Ito Y， et al. Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat[J］. Biol Pharm Bull， 1995，18(10)：1387.
[3］段智变，江汉糊，张书霞，等. 纳豆激酶粗制液对家兔溶血栓作用及其机制研究[J］. 营养学报，2003，25（1）：46.
[4］Fujita M，Hong K， Yae Ito，et al. Transport of nattokinase across the rat intestinal tract[J］. Niol Pharm Bull，1995，18(9)：1194.
[5］王萍，陈钧. 纳豆激酶纤溶酶活性研究[J］. 江西农业大学学报，2004，26（3）：431.
[6］Astrup T，Mullertz ST.The fibrin plate method for estimating fibrinolytic activity[J］. Arch Biochem Biophys，1952，40：346.
[7］中国药典2000年版.Ⅰ部[S］.2000：P504.
[8］Kunst F， Ogasawara N， Moszer I， et al. The complete genome sequence of the gram-positive bacterium Bacillus subtilis[J］. Nature，1997，390：249.
