Determination of carboplatin and pseudouridine in the human serum by HPLC

LI Jie;WNG Jing-ping;ZHOU Li-li;KONG Yong-xi;LIU Shu-zhe;WNG Ling

Chinese Pharmaceutical Journal ›› 2005, Vol. 39 ›› Issue (09) : 691-692.

Chinese Pharmaceutical Journal ›› 2005, Vol. 39 ›› Issue (09) : 691-692.
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Determination of carboplatin and pseudouridine in the human serum by HPLC

  • LI Jie1，WANG Jing-ping2， ZHOU Li-li1， KONG Yong-xia1， LIU Shu-zhe2， WANG Ling1
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Abstract

OBJECTIVE To establish a method for the determination of carboplatin and pseudouridine in human serum.METHODS The concentration of carboplatin and pseudouridine was assayed on a Spherigel-C18 column with a mobile phase consisting of acetonitrile-0.02 mol·L-1 KH2PO4(5:1 000)at a flow rate of 0.95 mL·min-1 and detected at 230 nm. RESULTS The calibration curve of carboplatin was linear over the range of 1～20 mg·L-1 (r= 0.990 2) .the average recovery of carboplatin was 97% . The limit of determination was 0.5 mg·L-1.The calibration curve of pseudouridine was linear over the range of 1～16 mg·L-1(r = 0.998 6). The average recovery of pseudouridine was 97% . The limit of determination was 0.3 mg·L-1. CONCLUSION This method is simple and highly stabile. Clinical application demonstrated that this method was practical.

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HPLC / carboplatin / pseudouridine

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LI Jie;WNG Jing-ping;ZHOU Li-li;KONG Yong-xi;LIU Shu-zhe;WNG Ling. Determination of carboplatin and pseudouridine in the human serum by HPLC [J]. Chinese Pharmaceutical Journal, 2005, 39(09): 691-692

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