Abstract
OBJECTIVE To study the effects of 3,4-oxo-isopropylidene shikimia acid(ISA) on the inflammatory reaction after cerebral ischemia and reperfusion in tMCAO(transient middle cerebral artery occlusion) rats, and its probable mechanism.METHODS The rats were subjected to MCAO for 3 h. After 21 h of reperfusion, TTC staining of brains was made to determine the size of cerebral infarction. The contents of IL-8,TNF-α, IL-1β and MPO in brain tissue were assayed. RESULTS After tMCAO, the vehicle group manifested the size of cerebral infarction as well as the content of TNF-α,IL-1β,IL-8 and MPO raised vs ISA group, ASA group and sham group. Above indicators were decreased significantly(P< 0.05 or 0.01 )in ISA 200,100 mg·kg-1 and ASA 50 mg·kg-1. CONCLUSION ISA can relieve the inflammatory reaction, namely leukocytic infiltrate and expression of inflammatory cytokine in brain tissue after cerebral ischemia and reperfusion, which may be an important mechanism for its resisting ischemic and reperfusion injury.
Key words
3 /
4-oxo-isopropylidene shikimic acid (ISA) /
middle cerebral artery /
cerebral ischemia and reperfusion /
inflammation /
myeloperoxidase(MPO) /
cytokine
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SUN Wen-yn;SUN Jin-ning;LIU Zhen-qun;HUNG Jin-ying;ZHNG Wen-o.
Effects of 3,4-oxo-isopropylidene shikimic acid (ISA) on the inflammatory reaction in rats subjected to middle cerebral artery ischemia and reperfusion and its probable mechanism [J]. Chinese Pharmaceutical Journal, 2005, 39(09): 678-680
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