OBJECTIVE A HPLC method was established for the determination of clindamycin phophate for clindamycin phophate suppositories METHODS Clindamycin phophate was separated on Symmetry Shield C₁₈ column (5μm, 250 mm×4.6 mm) with citric acid solution (citric acid 1.05 g dissolved in water and diluted to 1 000 mL)-acetonitrile (78∶22) as the mobile phase and detected at 210 nm. The flow rate was 1.0 mL·min^-1 RESULTS The standard curve was linear over the concentration range of 99.36～149.04 μg·mL^-1 and the correlation coefficient was 0.999 7. The average recovery and the relative standard deviation was 101.2%， and 0.25% respectively CONCLUSION The method is simple, rapid, accurate and reliable.