OBJECTIVE To prepare etoposide-loaded liposomes, determine its drug content and encapsulation efficiency, compare its in vitro release with that of etoposide power,and study its stability after sterilization METHODS The etoposide-loaded liposomes were prepared by film- ultrasonic wave dissolving techniques. The optimum formula was selected through uniform design. Dialytic method was used to determine drug content and encapsulation efficiency. Moist heat sterlization and60 Co radiation sterilization were applied. The vesicle size and percolation rate were used as stability parameters RESULTS The drug content of etoposide-loaded liposomes prepared by the optimum formular was (574.3±30.7)μg·mL-1. The average encapsulation efficiency was (61.58±0.83)%. The in vitro release of etoposide-loaded liposomes added up to (96.13±1.11)% in 50 h. The release of etoposide powder added up to (97.10±1.84)% in 3 h.The average percolation rate was higher with moist heat sterilization than with sterilization by60 Co radiation CONCLUSION The etoposide-loaded liposomes prepared by film- ultrasonic wave dissolving techniques were regular in morphology, and their vesicle sizes were small and even. The liposomes were conformed to be sustained-release preparation through in vitro release experiments. They were stable after60 Co radiation sterilization.
Key words
etoposide /
liposomes /
sterilization
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