OBJECTIVE To investigate the effects of triptolide on airway smooth muscle cells (ASMC) proliferation and expressions of oncogene c-fos and c-jun in culturing ASMC.METHOD S The primary ASMC from rat were collected and cultured and studied.The effects of triptolide at different doses (5,20,80,320 and 1 280 μg·L-1) and dexamethasone (1 mg·L-1) on ASMC proliferation were studied by MTT method.The mRNA expressions of c-fos and c-jun form ASMC were examined by RT-PCR.RESULTS The ASMC proliferation was inhibited at the dose (IC50=51.52 mg·L-1).The mRNA expression of c-fos and c-jun in ASMC was also inhibited by triptolide (80,320 and 1 280 μg·L-1) and dexamethasone.CONCLUSION S It suggested that triptolide remarkably inhibited ASMC proliferation probably by suppressing the expression of c-fos and c-jun.
Key words
triptolide /
oncogene /
asthma
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