Determination of toremifene citrate in plasma by HPLC

WNG Zhuo;ZHNG Li-mei;YNG Zhng-wei;XU Hui-xin;HU Jin-hong;ZHNG Xue-yn;LI Qing

Chinese Pharmaceutical Journal ›› 2003, Vol. 38 ›› Issue (02) : 121-123.

Chinese Pharmaceutical Journal ›› 2003, Vol. 38 ›› Issue (02) : 121-123.
临床药学

Determination of toremifene citrate in plasma by HPLC

  • WANG Zhuo1,ZHANG Li-mei2,YANG Zhang-wei1,XU Hui-xin1,HU Jin-hong1,ZHANG Xue-yan3,LI Qiang3
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Abstract

OBJECTIVE To establish a METHOD to determine the plasma drug concentrations of toremifene citrate in patients with nonsmall cell lung cancer as a multidrugresistant modulator.METHODS A C18(10 μm,150 mm×4.6 mm)column was used.The mobile phase was consisted of methnol100 μmol·L-1 ammonium acetatetriethylamine (90∶10∶0.05).The wavelength was 277 nm.RESULT The method was linear over the range of 0.1~10.0 μg·mL-1(r=0.999 9), and the precision and recoveries were good. It had been used successfully to determine clinical samples.CONCLUSION This method could be used to determine toremifene in plasma with highdose to reverse multidrugresistant in NSCLC patients.But there were differences between the concentrations measured and that reported by literature.

Key words

toremifene citrate / plasma drug concentration / high performance liquid chromatography / multidrugresistant / nonsmall cell lung cancer

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WNG Zhuo;ZHNG Li-mei;YNG Zhng-wei;XU Hui-xin;HU Jin-hong;ZHNG Xue-yn;LI Qing. Determination of toremifene citrate in plasma by HPLC[J]. Chinese Pharmaceutical Journal, 2003, 38(02): 121-123

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