Antioxidative activity of isorhapontigenin in vitro

WNG Qing-li;LIN Mo;LIU Geng-to;

Chinese Pharmaceutical Journal ›› 2001, Vol. 36 ›› Issue (12) : 810-814.

Chinese Pharmaceutical Journal ›› 2001, Vol. 36 ›› Issue (12) : 810-814.
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Antioxidative activity of isorhapontigenin in vitro

  • WANG Qing-li；LIN Mao；LIU Geng-tao；
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Abstract

OBJECTIVE To investigate the antioxidative activity of natural isorhapontigenin (ISOR) with similar chemical structure to resveratrol.METHODS The oxidative damage of rat liver microsomes, brain mitochondria and synaptosomes was induced by Fe2+-Cys, VitC-ADP-Fe2+ and H2O2,respectively.The formation of malondialdehyde (MDA), decrease of reduced glutathione (GSH) and increase of ultra-weak chemiluminescence during the lipid peroxidation process were determined．The characteristic ultra-weak chemiluminescence of oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2 system was also observed.RESULTS ISOR significantly inhibited MDA formation in liver microsomes, brain mitochondria and synaptosomes induced by Fe2+-Cys. Also,ISOR markedly prevented the decrease of GSH in mitochondria and synaptosomes induced by H2O2.The increase of ultra-weak chemiluminescence during lipid peroxidation induced by VitC-ADP-Fe2+ as well as oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2 were all inhibited by ISOR.The effects of ISOR 10-5 and 10-6 mol·L-1 on the MDA formation and decrease of GSH were corresponding to that of classical antioxidant vitamin E (10-4 mol·L-1).CONCLUSION ISOR possesses potent antioxidative activity and is much more potent than vitamin E.

Key words

isorhapontigenin / antioxidant / chemiluminescence / oxidative DNA damage

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WNG Qing-li;LIN Mo;LIU Geng-to;. Antioxidative activity of isorhapontigenin in vitro[J]. Chinese Pharmaceutical Journal, 2001, 36(12): 810-814

References

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