OBJECTIVE To establish an effective and validated method for determining the contents of aspirin and salicylic acid in low-dosage aspirin enteric-coated tablets.METHODS The C₁₈ column was used and the mobile phase was consisted of acetonitrile-THF-glacial acetic acid-water (23∶5∶5∶61) and the detection wavelength was 280 nm.RESULTS The interference from placebo was prevented by the RP-HPLC method.CONCLUSION The RP-HPLC assay was reliable for determing the contents of aspirin and salicylic acid in low-dosage aspirin enteric-coated tablets.