Preparative techniques and release properties of PLA and PLG microspheres containing tetanus toxoid protein

武汉生物制品研究所;湖北 武汉 000

Chinese Pharmaceutical Journal ›› 2001, Vol. 36 ›› Issue (05) : 318-322.

Chinese Pharmaceutical Journal ›› 2001, Vol. 36 ›› Issue (05) : 318-322.
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Preparative techniques and release properties of PLA and PLG microspheres containing tetanus toxoid protein

  • 武汉生物制品研究所,湖北 武汉 430060
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Abstract

OBJECTIVE To investigate the preparation and release properties of poly lactid acid(PLA) and poly lactidco glycolide acid (PLG) microspheres (MS) containing tetanus toxoid (TT) protein.METHOD The preparation property and optimum techniques of PLAMS were investigated by microscope and colorimetry using Even's blue, a low molecular weight bio dye as a model encapulating material.According to the optimum techniques,the microspheres containing TT protein were prepared.RESULT During the preparation of microspheres containing TT protein,the loss of TT protein from internal aqueous phase to external aqueous phase occurred and the loss of TT decreased when the concentration of polymer in dichlormethane increased to 25%~30%.The distribution of encapulated TT protein in microspheres was relevant with the weight distribution of different size of microspheres.In vitro,the peak release time of encapulated TT protein was delayed when the diameter of microspheres increased. CONCLUSION The TT proteinloading PLA and PLG microspheres with a smooth and nonporous surface was prepared by optimum techniques.The different size of microspheres containing TT protein can also be prepared for the need of controlled peak release time of encapulated TT protein in vitro and in vivo.

Key words

polymer microspheres / tetanus toxoid protein / properties

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武汉生物制品研究所;湖北 武汉 000. Preparative techniques and release properties of PLA and PLG microspheres containing tetanus toxoid protein[J]. Chinese Pharmaceutical Journal, 2001, 36(05): 318-322

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