OBJECTIVE To investigate the enhancing efffect of flexible nano-liposome on the transdermal delivery of cyclosporine.METHODS The effects of common nano-liposome,flexible nano-liposome containing sodium cholate and micelles on the penetration of cyclosporin A through excised mice skin were assessed by an in vitro permeation technique using modified Franz-diffusion cells.RESULTS When applied onto the mice skin non-occlusively,flexible nano-liposome promoted the transfer of cyclosporin A into the receiver.The permeation of cyclosporin A through per cm2 mice skin was 1.16 μg after 8h of transportation and amounted to 1.88 μg 24 h later.The residual amount in the skin was (1.78±0.51) μg·cm^-2.Common nano-liposome failed to transfer cyclosporin A into the receiver while promoted the accumulation of drug in the skin,up to (0.72±0.19) μg·cm^-2.1% and 40% Micelles precluded the transport of cyclosporin A into the mice skin.CONCLUSION Flexible nano-liposomes can be effective carriers for the transdermal delivery of cyclosporine.