OBJECTIVE To study the formulation and preparation of amphotericin B liposomes(LAMB),and evaluate its quality.METHOD The liposomes were prepared by an film-ultrasonic technique.The morphology of LAMB,the drug loading,the entrapment efficiency and the particle size distribution were studied.RESULTS More than 70% of initial amphotericin B content were entrapped into LAMB.The result of electron micrography and the size distribution showed that the LAMB were small spherical or similar to spherical vesicles,the mean diameter of LAMB was 79 nm with the range of 23～122 nm,with 86% of liposomes smaller than 96 nm.CONCLUSION Amphotericin B was highly entrapped into liposomes with good reproduction and stability.