OBJECTIVE This paper established a high-performance liquid chromatography for the determination of salbutamol (SBA) in human plasma using morphine as the internal standard. METHOD A variable wavelength UV detector and Ultraspher Cyano column (5μm, 250 mmx4.6 mm,Beckman)were used.To the plasma sample 1.0,0.5 ml phosphate buffer (2.0 mol·L-1 ,pH9.0) containing0.4% diphe-nylboric acid 2-aminoethyl ester was added, then extracted with 4.0 ml chloroform containing 1% tetraoctylammonium bromide. The organic layer was removed and extracted again with 200μl (0.08 mol·L-1) acetic acid. 100 of the acid layer was injected onto the column .The mobile phase of acetonitrile-methanol-pH2.8,0.025 mol? L_1 phosphate buffer (2:0.5:97.5) was. pumped at the rate of 1.0 ml?min-1 through the column. The detector at 0.002 AUFS was set at 224 nm. RESULTS The retention times for SBA and morphine were 7.0 min and 8.3 min,respectively.The standard curve was linear over the concentration range of 0.5 to 32 μg·L-1 .The detection limit in plasma was 0.3μg·L-1.Extraction recovery was 93% ~ 99% ；The method recovery was 98% ~ 109% ；with intra-day RSD less than 5% ；inter-day RSD less than 8% . CONCLUSION This method was found to be simple,rapid,sensitive and accurate for the determination of SBA in human plasma.