OBJECTIVE To estimate an HPLC for determination of gardenoside in Tuihuang oral liquid. METHODS Chromatographic olumn C₁₈ODS;Mobile phase MeOH-water 20∶80)；detection wavelength 240 nm. RESULTS The linear regression equation was Y=1566700x-4691,r=0.9998.The average recovery was 99.25% with RSD=2.78% n=5). CONCLUSION This is a simple,accurate method which can be used for quantitative analysis of Tuihuang oral liquid.