Determination of MS-551 concentration in plasma by HPLC
Meng Ling
Author information+
(Merig L)，Li Xiaoying (Li XY)，Liao Jie (Liao J
{{custom_zuoZheDiZhi}}
{{custom_authorNodes}}
{{custom_bio.content}}
{{custom_bio.content}}
{{custom_authorNodes}}
Collapse
History+
Received
Published
1998-04-28
1999-11-05
Issue Date
1999-11-05
Abstract
OBJECTIVE:To report a method for the determination of MS-551(MS) concentrations in plasma by HPLC.METHODS:MS was separated on a C18 column(5μm,4.6mm×15cm) by using methanol -0.1M ammonium acetate (60∶40) as mobile phase with detection wavelength at 270nm.Quantification was carried out based on the external standard of MS.RESULTS:There was a good linearity over the range from 0.1 to 2.0μg·ml-1 of MS,Y=8.6963×10-2+3.55×10-6X(r=0.9990).The average recovery was 94.27%.The detection limit was 0.85ng.At the same time,the relationship between the plasma concentrations of MS and QTc was determined in anaesthetized dogs with MS.CONCLUSION:This method is simple,practical and accurate.
Meng Ling .
Determination of MS-551 concentration in plasma by HPLC[J]. Chinese Pharmaceutical Journal, 1999, 34(11): 759-761
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
1 Katakami T，Yokoyama T，Miyamoto M，et al．Synthesis and pharmacologica studies of N-substituted 6-［(2-aminoethy)amino］ 1，3-dimethyl2；4(1H，3H)-pyrimidinediones，novel class Ⅲ antiarrhythmic agents．J Med Chem，1992，35(18)：3325．
2 Kamiya J，Ishii M，Katakami T．Antiarrhythmic effects of MS551，a new class Ⅲ antiarrhythrnic gaent，on canine models of ventricular arrhythmia．J pn J Pharmacol．1992，58(2)：112．
3 Kondoh K，Hashimoto H，Nishiyama H，et al．Effects of MS-551，a new class Ⅲ antiarrhythmic drug，on programmed stimulation-induced ventricular arrhythmias，electropysiology，and hemodynamics in a canine myocardial infarction model．J Cardiovasc Pharmacol，1994，23(4)：675．
4 Kamiya J，Ishii M，Yoshihara K，et al．MS-551：Pharmaoological profile of a novel class Ⅲ antiarrhythmie gaent．Drug Dev Res，1993，30(1)：39．
