Abstract
OBJECTIVE:To report a method for the determination of MS-551(MS) concentrations in plasma by HPLC.METHODS:MS was separated on a C18 column(5μm,4.6mm×15cm) by using methanol -0.1M ammonium acetate (60∶40) as mobile phase with detection wavelength at 270nm.Quantification was carried out based on the external standard of MS.RESULTS:There was a good linearity over the range from 0.1 to 2.0μg·ml-1 of MS,Y=8.6963×10-2+3.55×10-6X(r=0.9990).The average recovery was 94.27%.The detection limit was 0.85ng.At the same time,the relationship between the plasma concentrations of MS and QTc was determined in anaesthetized dogs with MS.CONCLUSION:This method is simple,practical and accurate.
Key words
MS-551 /
HPLC /
plasma concentration
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Meng Ling .
Determination of MS-551 concentration in plasma by HPLC[J]. Chinese Pharmaceutical Journal, 1999, 34(11): 759-761
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References
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