Determination of MS-551 concentration in plasma by HPLC

Meng Ling

Chinese Pharmaceutical Journal ›› 1999, Vol. 34 ›› Issue (11) : 759-761.

Chinese Pharmaceutical Journal ›› 1999, Vol. 34 ›› Issue (11) : 759-761.
药品质量及检验

Determination of MS-551 concentration in plasma by HPLC

  • Meng Ling
Author information +
History +

Abstract

OBJECTIVE:To report a method for the determination of MS-551(MS) concentrations in plasma by HPLC.METHODS:MS was separated on a C18 column(5μm,4.6mm×15cm) by using methanol -0.1M ammonium acetate (60∶40) as mobile phase with detection wavelength at 270nm.Quantification was carried out based on the external standard of MS.RESULTS:There was a good linearity over the range from 0.1 to 2.0μg·ml-1 of MS,Y=8.6963×10-2+3.55×10-6X(r=0.9990).The average recovery was 94.27%.The detection limit was 0.85ng.At the same time,the relationship between the plasma concentrations of MS and QTc was determined in anaesthetized dogs with MS.CONCLUSION:This method is simple,practical and accurate.

Key words

MS-551 / HPLC / plasma concentration

Cite this article

Download Citations
Meng Ling . Determination of MS-551 concentration in plasma by HPLC[J]. Chinese Pharmaceutical Journal, 1999, 34(11): 759-761

References

1 Katakami T,Yokoyama T,Miyamoto M,et al.Synthesis and pharmacologica studies of N-substituted 6-[(2-aminoethy)amino] 1,3-dimethyl2;4(1H,3H)-pyrimidinediones,novel class Ⅲ antiarrhythmic agents.J Med Chem,1992,35(18):3325. 2 Kamiya J,Ishii M,Katakami T.Antiarrhythmic effects of MS551,a new class Ⅲ antiarrhythrnic gaent,on canine models of ventricular arrhythmia.J pn J Pharmacol.1992,58(2):112. 3 Kondoh K,Hashimoto H,Nishiyama H,et al.Effects of MS-551,a new class Ⅲ antiarrhythmic drug,on programmed stimulation-induced ventricular arrhythmias,electropysiology,and hemodynamics in a canine myocardial infarction model.J Cardiovasc Pharmacol,1994,23(4):675. 4 Kamiya J,Ishii M,Yoshihara K,et al.MS-551:Pharmaoological profile of a novel class Ⅲ antiarrhythmie gaent.Drug Dev Res,1993,30(1):39.

Accesses

Citation

Detail

Sections
Recommended

/