Study on the percutaneous absorption of recombination human epidermal growth factor
Jiang Guohui
Author information+
(Jiang GH)，Tang Ganghua (Tang GH)，Miao Xiaochun (Miao xC)，et a
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History+
Received
Published
1998-07-06
1999-11-05
Issue Date
1999-11-05
Abstract
OBJECTIVE:To study the absorption,permeation and metabolism of epidermal growth factor (EGF) in the surface of the skin.METHODS:125I-EGF was labelled with Chloramine-T method and a Franz diffusion cell syntem was developed for the percutaneous absorption study.The accumulated permeation amount and ratio of 125I-EGF at the different time were determined by the direct radioisotope tracing assay and the trichloroacetic acid (TCA) precipitation method.RESULTS:The total accumulated permeability ratios during 12h were all less than 1% at the different dose of 125I-EGF.There was no linearity between the dose of 125I-EGF smeared on the surface of the skin and the accumulated permeability ratio. CONCLUSION:The study provided necessary imformation for further clinical trial.
Jing Guohui .
Study on the percutaneous absorption of recombination human epidermal growth factor[J]. Chinese Pharmaceutical Journal, 1999, 34(11): 746-749
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