OBJECTIVE:To establish a quantitative method for the determination of two main active ingredients,evodiamine and rutaecarpine,in Chinese medicinal crop Evodia rutaecarpaand its preparation Huatuo Zaizao pill.METHOD:Evodiamine and rutaecarpine were determined by RP-HPLC for Evodia rutaecarpa,the mobile phase consisted of acetonitrile-water(50∶50),the flow rate was 1.0ml·min^-1,medroxyprogesteron acetate was used as internal standard.For Huatuo Zaizao pill,the mobile phase consisted of acetonitrile-water(40∶60),the flow rate was 1.0ml·min^-1,biphenyl was used as internal standard.The eluent was monitored with UV detector at225nm.RESULTS:For Evodia rutaecarpa,the standard curves of evodiamine and rutaecarpine were all linear over the concentration range of 5～50μg·min^-1.The average recoveries were 96.6% and 96.4% respectively.The intra-run relative standard deviations were 0.8%～1.5% and 1.1%～2.3%(n=6),respectively,the inter-run relative standard deviations were 2.3%～3.6% and 2.5 %～3.9%(n=6),respectively.For Huatuo Zaizao pill,the standard curve of evodiamine and rutaecarpine were all linear over the range of 5～20μg·ml^-1,the average recoveries were 97.6% and 94.6%,respectively.The contents of evodiamine and rutaecarpine in Evodia rutaecarpa from four localities of growth were 0.118% and 0.092%,0.213% and 0.106%,0.581% and 0.609%,0.4 78% and 0.459%,respectively.In Huatuo Zaizao pill,the contents of two ingredients were 0.1123mg·g^-1 and 0.0981mg·g^-1,respectively.CONCLUSION:This method is accurate,convenient and sensitive.It is satisfactory for the quality control of Evodia rutaecarpa and its preparations.