OBJECTIVE:To study on the pharmacokinetics of Mitomycin C (MMC) penetrated into the pancereatic juice.METHODS:6 mongrel dogs following 2 mg single dose by intravenous injection were enrolled after operated pancreatico drainage tube by surgery.MMC concentrations in plasma and pancreatic juice were determined by using a rapid and sensitive RP-HPLC assay.Pharmacokinetic behavious of MMC in plasma and pancreatic juice were analysed by using computer program PCNONLIN (SCI,v.4.2).RESULTS:PK of MMC in plasma and pancreatic juice were compatible with a two-compartment open model.Pharmacokinetic parameters in plasma and pancreatic juice were t_max=(0.36±0.16)h and c_max=(65.6±75.8)μg·L^{-1for pancreatic juice,t_1/2βwere(1.17±0.96)h and (0.71±0.66)h,and AUC_0～∞(107.9±43.9)μg·h·L-1 and (33.5±28.8)μg·h·L-1,respectively.CONCLUSION:MMC penetrated rapidly into pancreatic juice after iv,and was eliminated fast from pancrease than plasma (P<0.01).The mean penetration index (PI) of MMC was (0.31±0.19).}