A sensitive method for the determination of homocysteine by HPLC

Jie Xinming

Chinese Pharmaceutical Journal ›› 1999, Vol. 34 ›› Issue (05) : 324-326.

Chinese Pharmaceutical Journal ›› 1999, Vol. 34 ›› Issue (05) : 324-326.
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A sensitive method for the determination of homocysteine by HPLC

  • Jie Xinming
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Abstract

OBJECTIVE:To establish a sesitive method to determine homocysteine by a reversed-phase high-performance liquid chromatography (RP-HPLC).METHODS:Homocysteine was determined by RP-HPLC with Fluorescence spectrophotometry.RESULTS:Ophthaladehyde was used as fluorescent derivative reagent.When the reaction took place at 50℃ and pH8.4 for 4 min,the fluorescent intensity reached the highest.In the concentraction of 5×10-9～2.7×10-7mol·L-1,F%=6.52×109c-0.91,r=0.998,RSD=2.24%,detection limit=4.2×10-10mol·L-1.CONCLUSION:Treated with H2O2,the fluorescent intensity of ophthalaldehyde derivatives of HCY may increase by 5 times.

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homocyteine / HPLC / cardiovascular disease

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Jie Xinming. A sensitive method for the determination of homocysteine by HPLC[J]. Chinese Pharmaceutical Journal, 1999, 34(05): 324-326

References

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