OBJECTIVE: To study pharmaceutical regularity of percutaneous absorption. METHODS: The percutaneous absorption speed of one hundred drugs and the different permeation enhancing activity of 2% and 5% Azone were studied in mouse with improved Fick's diffusing installation by computing accumulative permeation quantity (Q), steady percutaneous speed (J), and permeation coefficient (Kp). RESULTS AND CONCLUSION: We discussed the rules in pharmaceutics of drug's physical and chemical characteristics and peucutaneous absorption, calculated the experimental regressive equation of drug percutaneous absorption and compared the influence of different concentration Azone to drug percutaneous permeation and equation.