OBJECTIVE: To stablish TLC-densitometry of alismol in Rhizoma Alismatis. METHODS: The samples were extracted by CC(hexane - CH2 Cl2 ,1:1)method and subjected to TLC-densitometry ( coloured by silimtungstic acid). RESULTS: The alismol content in Rhizoma Alismatis was 0.021%and RSD was 4.1%.CONCLUSION Alismol was efficiently quantitated by TLC-densitometry.
Key words
Alisma orientalis /
alismol /
TLC-densitometry
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References
1 Kato T, Tomita M, Takigana M. et al, Inhibitory effects and active constituents of Alisma Rhiwmes on vascular contraction induced by high consentranon on KCI. Bull Chem Soc Jpu 1994,67:1394.
2 Yamahara J, Kobayashi G, Iwamoto M. et al, The effect of alismol isolated from Alisma orientalis on experimental hypertensive models in rats. Phytotherapy Research，1989,3:57.
3 Matsuda H, Kobayashi G, Yamahara J. et al, Quantitative Analysis of Alismol in Alismatis Rhizoma. Shoyakugaku Zasshi, 1988, 42: 147.
4 Nakajima Y, Satoh Y, Katsumata M, et al,Terpenoids of Alisma orientate rhizome and the crude drug alismatis rhizoma.Pytochemistry, 1994,36:119.
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Footnotes
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