OBJECTIVE:To explore the mechanism of epilepsy evoked by coriaria lactone (CL), we studied the effects of CL on glutamate(Glu) release and uptake. METHOD:Radioisotope technique. RESULTS: CL(0.35一350 pmol · L-1)enhanced the Glu release from the cerebral slies in the depolarization evoked by 50 mmol·L-1KCl. The increase rates were 11 .2%，16.7%，27.4%，33.2 % respectively. The relationship of dose and effect was obvious, but no effect was observedin the rest state. The Glu uptake of synaptosomes was decreased by CL in the concentration of 350 }mol-1，with a decrease rate of 7.96%，but no effect was observed in other concentrations. CONCLUSION: CL enhanced Glu release in the depolarizing state and decreased Glu uptake.in the rest state. The Glu uptake of synaptosomes was decreased by CL in the concentration of 350 }mol-1，with a decrease rate of 7.96%，but no effect was observed in other concentrations. CONCLUSION: CL enhanced Glu release in the depolarizing state and decreased Glu uptake.
Key words
coriaria lactone /
glutamate /
uptake /
release
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References
1 吴良芳，廖德阳，李秀琼，等.大鼠马桑内酯全身型点燃效应癫痫模型.华西医科大学学报.1987,18(2):104.
2郭亮.谢杨高，柴慧霞.马桑内酯在大鼠离体海马脑片上引起的癫痫样放电活动.华西医科大学学报.1990, 21(1):50.
3 Patterson T', Dawson Jr R. Inhibition of trimethylin-stimulated glutamate(Glu) release by dilfiazem. Faseb J,1990,7(4):4935.
4 Lowry OH, Rosebrough NJ, Farr AL. Protein measuremeat with the folin phenol reagent. J Bio Chem, 1951, 193:265.
5 Maycox PR, Deckwerth T, Hell JW, et al .Glutamate uptake by brain synaptic vesicles. J Biol Chem, 1988, 263:15423.
6 Carlson H, Ronne Engstrom E, Ungerstedt V, et al .Seizure related elevations of extracellular amino acid in human focal epilepsy. Neurosci Lett ,1992,140(1) : 30.
7 Nicholls DG. The release of glutamate, aspartate and GABA from isolated nerve terminals. J Neurochem，1989,52:331.
8 McMahon HT, Nicholls DG. Transmitter glutamate release from isolated nerve terminals. J Neurochem, 1991, 56:86.
9 Leone AM, Palmer RMJ, Knowles RG, et al. Constitutive and inducible nitric oxide synthase incorporate molecular oxygen into both nitric oxide and citrulline. J Biol Chem,1991,266:23790.
10 McMahon HT, Barrie AP, Loew M, et al . Glutamate release from guinea pig synaptosomes. J Neurochem, 1989, 53:71.
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