Determination of sinomenine in serum by thin-layer chromatography using solid phasc extraction

He Lngchong

Chinese Pharmaceutical Journal ›› 1997, Vol. 32 ›› Issue (02) : 110-112.

Chinese Pharmaceutical Journal ›› 1997, Vol. 32 ›› Issue (02) : 110-112.
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Determination of sinomenine in serum by thin-layer chromatography using solid phasc extraction

  • He Langchong
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Abstract

OBJECTIVE: To study the purification of sinomenine in serum by the liquid-solid extraction and the pharmacokinetics of sinomenine in rabbits. METHODS: To sinomenine in serum was extracted by silica column which made in our laboratory and redissolved in CHC13 .The sample was applied on silica GF254 plate and developed with CHCI3- CH3OH(19:2).The spot was detected by CS-930 atλs=275 m,λR=320 nm. RESULTS: The average recovery was 96.95%±s7.59%for SPE(solid phase extraction) procedure in serum. The drug-time curve of sinomenine in rabbitswas determined. CONCLUSION: The SPE columns which made in our laboratory are simple and efficient for the purification of serum samples. The TLC is sensitive and precise for the determination of sinomenine.

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smomenme / serum chromatography

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He Lngchong. Determination of sinomenine in serum by thin-layer chromatography using solid phasc extraction[J]. Chinese Pharmaceutical Journal, 1997, 32(02): 110-112

References

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